酿酒酵母中角鲨烯环氧化酶形成和活性的氧气需求。
Oxygen requirements for formation and activity of the squalene epoxidase in Saccharomyces cerevisiae.
作者信息
Jahnke L, Klein H P
出版信息
J Bacteriol. 1983 Aug;155(2):488-92. doi: 10.1128/jb.155.2.488-492.1983.
Abstract
The effect of oxygen on squalene epoxidase activity in Saccharomyces cerevisiae was investigated. In cells grown in standing cultures, the epoxidase was localized mainly in the "mitochondrial" fraction. Upon aeration, enzyme activity increased and the newly formed enzyme was associated with the "microsomal" fraction. At 0.03% (vol/vol) oxygen, epoxidase levels doubled, whereas the ergosterol level was only slightly increased. Cycloheximide inhibited the increase in epoxidase under these conditions. An apparent Km for oxygen of 0.38% (vol/vol) was determined from a crude particulate preparation for the epoxidase.
摘要
研究了氧气对酿酒酵母中角鲨烯环氧化酶活性的影响。在静置培养的细胞中，环氧化酶主要定位于“线粒体”部分。通气后，酶活性增加，新形成的酶与“微粒体”部分相关。在0.03%（体积/体积）氧气浓度下，环氧化酶水平翻倍，而麦角固醇水平仅略有增加。在这些条件下，放线菌酮抑制了环氧化酶的增加。从粗制颗粒制剂中测定环氧化酶的氧气表观Km为0.38%（体积/体积）。
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