Issekutz A C
J Immunol Methods. 1983 Jul 29;61(3):275-81. doi: 10.1016/0022-1759(83)90221-1.
Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, it is often necessary to eliminate even minute quantities of endotoxin from such solutions. A process is described which exploits the high binding affinity of polymyxin B for the lipid A moiety of most endotoxins in order to remove endotoxins from solutions by chromatography on polymyxin B Sepharose 4B. This method was simple, very effective, resulting in essentially complete removal of several endotoxins from heavily contaminated solutions (1-10 micrograms/ml by Limulus amoebocyte lysate assay) and employed mild physiological conditions.
革兰氏阴性菌释放的内毒素是水性溶液和生理溶液中常见的污染物。由于它们在体内和体外具有强大的生物学效应，通常有必要从这类溶液中去除哪怕极微量的内毒素。本文描述了一种利用多粘菌素B对大多数内毒素脂质A部分的高结合亲和力，通过在多粘菌素B琼脂糖4B上进行色谱分离从溶液中去除内毒素的方法。该方法简单、非常有效，能从严重污染的溶液（通过鲎试剂检测为1 - 10微克/毫升）中基本完全去除几种内毒素，并且采用温和的生理条件。
