Dérer M, Walker C, Kristensen F, Reinhardt M C
J Immunol Methods. 1983 Jul 29;61(3):359-65. doi: 10.1016/0022-1759(83)90232-6.
A new method for measuring uptake of baker's yeast (BY) by human polymorphonuclear leukocytes (PMN) using flow cytometry is described. The method correlates excellently with the visual method, is reproducible and provides a means for investigating the early phases of the phagocytic process as well as the phagocytic capacity of PMN. This quick and accurate method allows the counting of large numbers of cells, and monitoring of the process of particle uptake and has a considerable potential in the routine assessment of polymorph function in various clinical situations.
描述了一种使用流式细胞术测量人类多形核白细胞（PMN）摄取面包酵母（BY）的新方法。该方法与视觉方法相关性极佳，具有可重复性，并为研究吞噬过程的早期阶段以及PMN的吞噬能力提供了一种手段。这种快速准确的方法能够对大量细胞进行计数，并监测颗粒摄取过程，在各种临床情况下对多形核功能的常规评估中具有相当大的潜力。
