Wilson R M, Galvin A M, Robins R A, Reeves W G
J Immunol Methods. 1985 Feb 11;76(2):247-53. doi: 10.1016/0022-1759(85)90301-1.
A new method for the measurement of phagocytosis of Candida albicans by human polymorphonuclear leucocytes (PMN) is described using a fluorescence activated cell sorter. We have used acridine orange to discriminate between PMN which have internalised yeast particles and those which have not. This method allows accurate measurement of particle phagocytosis as an event distinct from particle adherence. It also permits detailed examination of the kinetics of phagocytosis, the study of which is likely to be of value in the investigation of diseases where abnormalities of PMN function are suspected.
描述了一种使用荧光激活细胞分选仪测量人类多形核白细胞（PMN）对白色念珠菌吞噬作用的新方法。我们使用吖啶橙来区分已内化酵母颗粒的PMN和未内化的PMN。该方法能够准确测量颗粒吞噬作用，将其作为一个与颗粒黏附不同的事件。它还允许对吞噬作用的动力学进行详细研究，对其研究可能在怀疑PMN功能异常的疾病调查中具有价值。
