在大肠杆菌中合成具有人白细胞干扰素活性的多肽。
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
作者信息
Nagata S, Taira H, Hall A, Johnsrud L, Streuli M, Ecsödi J, Boll W, Cantell K, Weissmann C
出版信息
Nature. 1980 Mar 27;284(5754):316-20. doi: 10.1038/284316a0.
DOI:10.1038/284316a0
PMID:6987533
Abstract
Double-stranded cDNA prepared from the 12S fraction of poly(A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.
摘要
从产生干扰素（IF）的人白细胞的聚腺苷酸（poly(A)）RNA的12S组分制备的双链互补DNA（cDNA），使用pBR322载体克隆于大肠杆菌中。所得到的一个克隆含有一个910个碱基对的插入片段，它能与IF信使核糖核酸（mRNA）杂交，并负责产生具有生物学IF活性的一种多肽。从一些克隆中每克细胞可获得高达10,000单位的IF活性。
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