Novick D, Eshhar Z, Gigi O, Marks Z, Revel M, Rubinstein M
J Gen Virol. 1983 Apr;64 (Pt 4):905-10. doi: 10.1099/0022-1317-64-4-905.
Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN-beta 1) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN-beta 1. Electrophoretically pure IFN-beta 1, having a specific activity of 3 X 10(8) to 6 X 10(8) units/mg was obtained by a single passage of partially purified IFN-beta 1 on these immunoadsorbents.
通过一种基于用第二抗体进行免疫沉淀以及从免疫沉淀物中回收干扰素活性的新筛选程序，鉴定出了八种分泌对人成纤维细胞干扰素（IFN-β1）具有特异性的单克隆抗体的杂交瘤。由这些单克隆抗体制备了免疫吸附剂，并用于纯化IFN-β1。通过将部分纯化的IFN-β1在这些免疫吸附剂上单次通过，获得了比活性为3×10⁸至6×10⁸单位/毫克的电泳纯IFN-β1。
