Fromtling R A, Kaplan A M, Shadomy H J
Sabouraudia. 1983 Jun;21(2):113-9.
Immune responses, including lymphocyte transformation and protection, were evaluated in a new model of experimental murine cryptococcosis. Newly developed, genetically stable, acapsular mutants of Cryptococcus neoformans were used to immunize C3H/HeN mice. Intraperitoneal and subcutaneous routes of immunization without adjuvant were studied. Spleen cells and pooled popliteal and inguinal lymph node cells responded significantly to heat-killed, whole-cell C. neoformans antigen but not to Candida albicans antigen in lymphocyte transformation assays. No significant difference in lymphocyte transformation response to antigens was observed between intraperitoneally and subcutaneously immunized animals. Mean survival time of immunized mice was extended significantly in comparison to controls following intravenous challenge with virulent C. neoformans. Degree of protection was independent of route of immunization but dependent upon the magnitude of the challenge inoculum. This model should prove valuable in the study of immunoregulation of cryptococcosis and vaccine development.
在一种新的实验性小鼠隐球菌病模型中评估了免疫反应，包括淋巴细胞转化和保护作用。使用新开发的、基因稳定的新型隐球菌无荚膜突变体免疫C3H/HeN小鼠。研究了无佐剂的腹腔内和皮下免疫途径。在淋巴细胞转化试验中，脾细胞以及汇集的腘窝和腹股沟淋巴结细胞对热灭活的全细胞新型隐球菌抗原有显著反应，但对白色念珠菌抗原无反应。在腹腔内和皮下免疫的动物之间，未观察到对抗原的淋巴细胞转化反应有显著差异。在用强毒新型隐球菌静脉攻击后，免疫小鼠的平均存活时间与对照组相比显著延长。保护程度与免疫途径无关，但取决于攻击接种物的大小。该模型在隐球菌病免疫调节研究和疫苗开发中应具有重要价值。
