Murphy J W, Moorhead J W
J Immunol. 1982 Jan;128(1):276-83.
By injecting cryptococcal antigen i.v. into CBA/J mice, we have simulated a level of antigenemia that is known to occur naturally in murine and human cryptococcosis. The cryptococcal antigen induced a population of lymph node (LN) cells that, upon adoptive transfer, could specifically suppress the delayed-type hypersensitivity (DTH) response to cryptococcal antigen and the splenic T lymphocytes responsible for in vitro inhibition of growth of Cryptococcus neoformans. The cryptococcal-antigen-induced suppressor cell could be detected in the LN as early as 5 days and was still in significant numbers 7 days after antigen injection. Suppressor cells were not found in the spleens of tolerized mice, nor could serum from these animals adoptively transfer suppression. The suppressor cells in this model were shown to be T lymphocytes, which exerted their effect on the afferent limb of the DTH response; therefore, they were similar to the Ts1 cell in the ABA-DTH, NP-DTH, and NP cutaneous sensitivity models and to the afferent suppressor cells in the DNFB contact sensitivity system. The percent suppression of the cryptococcal DTH response was dependent on the amount of antigen injected. Approximately, 35 micrograms of cryptococcal antigen per ml of serum was sufficient to induce significant suppression of the cell-mediated immune response.
通过向CBA/J小鼠静脉注射隐球菌抗原，我们模拟了已知在鼠类和人类隐球菌病中自然发生的抗原血症水平。隐球菌抗原诱导了一群淋巴结（LN）细胞，这些细胞在过继转移后能够特异性抑制对隐球菌抗原的迟发型超敏反应（DTH）以及负责体外抑制新型隐球菌生长的脾T淋巴细胞。早在抗原注射后5天就能在LN中检测到隐球菌抗原诱导的抑制细胞，并且在注射抗原7天后其数量仍然可观。在耐受小鼠的脾脏中未发现抑制细胞，这些动物的血清也不能过继转移抑制作用。该模型中的抑制细胞被证明是T淋巴细胞，它们对DTH反应的传入支发挥作用；因此，它们类似于ABA-DTH、NP-DTH和NP皮肤敏感性模型中的Ts1细胞以及DNFB接触敏感性系统中的传入抑制细胞。隐球菌DTH反应的抑制百分比取决于注射的抗原量。每毫升血清中约35微克的隐球菌抗原足以诱导对细胞介导免疫反应的显著抑制。
