Lucius R, Rauterberg E W, Diesfeld H J
Tropenmed Parasitol. 1983 Jun;34(2):133-6.
Sodiumdodecylsulphate (SDS)-solubilized proteins of several developmental stages of Dipetalonema viteae (adults, uterus content, microfilariae, infective larvae) were subjected to SDS-polyacrylamide gel electrophoresis and transferred onto nitrocellulose filter sheets. Their antigenicity in natural infections of Meriones unguiculatus was investigated by immuno-recognition with pooled sera of infected hosts. The antibodies were labelled by radioiodinated protein A and visualized by autoradiography. Immunorecognized protein bands were demonstrable in all stages, some of them being stage specific, others common to several or all stages. The highest number of labelled protein bands was found in extracts of adult worms, the lowest in preparations of microfilariae and infective larvae.
将双瓣旋尾丝虫（成虫、子宫内容物、微丝蚴、感染性幼虫）几个发育阶段的十二烷基硫酸钠（SDS）增溶蛋白进行SDS聚丙烯酰胺凝胶电泳，并转移到硝酸纤维素滤膜上。用感染宿主的混合血清通过免疫识别研究它们在自然感染长爪沙鼠中的抗原性。抗体用放射性碘化蛋白A标记，通过放射自显影进行可视化。在所有阶段都能检测到免疫识别的蛋白条带，其中一些是阶段特异性的，其他的则是几个或所有阶段共有的。在成虫提取物中发现的标记蛋白条带数量最多，在微丝蚴和感染性幼虫制剂中最少。
