Prüsse A, Vollmer S, Diesfeld H J
Tropenmed Parasitol. 1982 Mar;33(1):33-6.
The antigenic properties of adult male and female worms of the nematode Dipetalonema viteae (Filarioidea) were studied by immunocytochemistry. Using sera of rodents infected with this parasite, the binding of antibodies to sections of worms embedded in Epon was assayed by the peroxidase-antiperoxidase (PAP) technique and by light microscopy. In female worms antigen-antibody reactions were located in the uterine fluid, the inner uterus wall, the circumference of the microfilariae and in the egg shell. Male D. viteae showed binding of antibodies in the vesica seminalis and in the vas deferens. Both sexes has also less pronounced antigenic activities in cuticula, muscle and intestine.
利用免疫细胞化学方法研究了丝状线虫类的成年雄性和雌性魏氏双瓣线虫的抗原特性。使用感染该寄生虫的啮齿动物血清，通过过氧化物酶-抗过氧化物酶（PAP）技术和光学显微镜检测抗体与包埋在环氧树脂中的虫体切片的结合情况。在雌虫中，抗原-抗体反应位于子宫液、子宫内壁、微丝蚴周围和卵壳中。雄虫的精囊和输精管中有抗体结合。两性的表皮、肌肉和肠道中也有较弱的抗原活性。
