Swerdlow M A, Chowdhury L N
Am J Clin Pathol. 1983 Sep;80(3):283-9. doi: 10.1093/ajcp/80.3.283.
Examination of liver biopsy specimens from 59 patients with alcoholic liver disease and 21 nonalcoholics by immunofluorescence and immunoperoxidase methods using fluorescein-conjugated and peroxidase-labeled antisera against IgA, IgA1, IgA2, and S-IgA showed that 47 of 59 biopsy specimens from alcoholics and 0 of 21 from non-alcoholics showed a "continuous" pattern of IgA and IgA-subclass deposition (P less than 0.001). Grading the intensity of immunofluorescence on 1-4 scale, biopsies with the continuous pattern showed grade 3-4 activity against IgA2 and S-IgA and only grade 1-2 activity against IgA1. Biopsies with the discontinuous pattern showed only grade 1-2 activity against S-IgA, IgA2, and IgA1. Grade 3-4 activity was persistent in all the 47 specimens with the continuous pattern, despite pretreatment with blocking anti-IgA1, whereas 20 and 38 biopsies showed the same activity after blocking with anti-IgA2 and anti-S-IgA sera, respectively. It is concluded from these studies that IgA2 subclass formed a major subclass component contributing to the continuous pattern of IgA deposition in hepatic tissues and that the major source for this IgA in alcoholics was probably derived from the gastrointestinal tract.
运用针对IgA、IgA1、IgA2和分泌型IgA（S-IgA）的异硫氰酸荧光素结合抗血清和过氧化物酶标记抗血清，通过免疫荧光和免疫过氧化物酶方法，对59例酒精性肝病患者和21例非酒精性肝病患者的肝活检标本进行检测，结果显示，59例酒精性肝病患者的活检标本中有47例，21例非酒精性肝病患者的活检标本中无一例呈现IgA及其亚类沉积的“连续”模式（P＜0.001）。按照1-4级对免疫荧光强度进行分级，呈现连续模式的活检标本针对IgA2和S-IgA显示3-4级活性，而针对IgA1仅显示1-2级活性。呈现不连续模式的活检标本针对S-IgA、IgA2和IgA1仅显示1-2级活性。在所有47例呈现连续模式的标本中，尽管用封闭性抗IgA1进行预处理，但3-4级活性依然持续存在；而在用抗IgA2和抗S-IgA血清封闭后，分别有20例和38例活检标本显示相同活性。从这些研究得出结论，IgA2亚类是肝组织中IgA沉积连续模式的主要亚类成分，并且酒精性肝病患者体内这种IgA的主要来源可能是胃肠道。
