Holdsworth S R, Wischusen N J, Dowling J P
Aust N Z J Med. 1983 Feb;13(1):15-20. doi: 10.1111/j.1445-5994.1983.tb04539.x.
A fluid phase radioimmunoassay (RIA) has been established for the detection of circulating anti-glomerular basement membrane (GBM) antibodies. Antibodies were detected by binding with a collagenase solubilised, immunochemically purified, radiolabelled extract of human particulate GMB. Sera from 108 patients with a variety of histological types of glomerulonephritis (GN) were assessed for the presence of antibodies. Likely positive sera were those from patients with clinical features of rapidly progressive glomerulonephritis (RPGN) with crescentic or diffusely proliferative GN and linear staining of their GBM's with IgG. Thirty one of 35 sera from such patients were positive in the RIA. Sera from 14 patients with RPGN but with granular IgG deposition (i.e. immune complex related GN) were all negative for anti-GBM antibodies as were sera from 59 patients with a variety of non-crescentic non RPGN without linear IgG. Comparison of the RIA with conventional indirect immunofluorescence (IIF) showed that the RIA had a higher detection rate of antibodies in likely positive sera (19/21 RIA: 16/21 IIF) lower false positive detection rate with sera from patients without GN (0/26 RIA: 2/26 IIF) and was three to four times more sensitive in serial dilution studies.
已建立一种液相放射免疫测定法（RIA）来检测循环抗肾小球基底膜（GBM）抗体。通过与胶原酶溶解、免疫化学纯化、放射性标记的人颗粒性肾小球基底膜提取物结合来检测抗体。对108例患有各种组织学类型肾小球肾炎（GN）的患者血清进行抗体检测。可能呈阳性的血清来自具有快速进行性肾小球肾炎（RPGN）临床特征且伴有新月体或弥漫性增殖性GN以及肾小球基底膜IgG线性染色的患者。此类患者的35份血清中有31份在RIA中呈阳性。14例RPGN但伴有颗粒状IgG沉积（即免疫复合物相关GN）患者的血清以及59例各种非新月体性非RPGN且无IgG线性沉积患者的血清，抗GBM抗体均为阴性。RIA与传统间接免疫荧光法（IIF）的比较表明，RIA在可能呈阳性的血清中抗体检测率更高（RIA为19/21，IIF为16/21），在无GN患者血清中的假阳性检测率更低（RIA为0/26，IIF为2/26），并且在系列稀释研究中灵敏度高出三到四倍。
