Bernard A M, Foidart J M, Mahieu P, Viau C, Lauwerys R R
Clin Chem. 1986 Aug;32(8):1468-72.
We describe a latex particle agglutination assay for detecting circulating antibodies against laminin, a noncollagenous glycoprotein of basement membranes. Polystyrene latex particles on which laminin has been adsorbed are incubated with serum for about 25 min at 42-45 degrees C. The agglutination is then measured by counting residual unagglutinated particles. Polyethylene glycol 6000 enhances the agglutination. The assay is fully automated, yielding results in about 45 min, for 50 samples per hour. Addition of purified laminin abolishes the agglutination of laminin-coated particles in practically all positive sera. The anti-laminin antibody titers obtained by this latex immunoassay and by radioimmunoassay correlated well in 161 sera from patients with suspected or established renal diseases. The agglutination assay more frequently gave positive results for cases of glomerulonephritis with linear deposits (20/22 cases) than for glomerulonephritis with granular deposits (7/68) or glomerulonephritis with no glomerular deposits (2/13). The finding of low anti-laminin antibody titers in sera from about 15% (34/230) of the healthy subjects suggests that these autoantibodies are pathogenic only in certain circumstances.
我们描述了一种用于检测抗层粘连蛋白循环抗体的乳胶颗粒凝集试验，层粘连蛋白是基底膜的一种非胶原糖蛋白。将吸附了层粘连蛋白的聚苯乙烯乳胶颗粒与血清在42 - 45摄氏度下孵育约25分钟。然后通过计数残留的未凝集颗粒来测量凝集情况。聚乙二醇6000可增强凝集作用。该试验完全自动化，每小时可处理50个样本，约45分钟得出结果。加入纯化的层粘连蛋白几乎可消除所有阳性血清中层粘连蛋白包被颗粒的凝集。通过这种乳胶免疫测定法和放射免疫测定法获得的抗层粘连蛋白抗体滴度在161例疑似或确诊肾病患者的血清中具有良好的相关性。对于有线性沉积物的肾小球肾炎病例（20/22例），凝集试验比有颗粒状沉积物的肾小球肾炎（7/68例）或无肾小球沉积物的肾小球肾炎（2/13例）更常得出阳性结果。在约15%（34/230）的健康受试者血清中发现低抗层粘连蛋白抗体滴度，这表明这些自身抗体仅在某些情况下具有致病性。
