Post G B, Keller D A, Connor K A, Menzel D B
Biochem Biophys Res Commun. 1983 Jul 29;114(2):737-42. doi: 10.1016/0006-291x(83)90842-2.
The effects of varying culture conditions on glutathione content in A549 (human type II lung tumor derived) cells were examined. Parameters studied were growth time, serum concentration, and the presence or absence of a mixture of insulin, transferrin, and selenous acid. Glutathione content increased with serum concentration. When cells were grown with serum, glutathione increased sharply 24 hours after passage and decreased thereafter. Insulin, transferrin, and selenous acid had little effect on cell growth or glutathione content. Replacement of media with fresh media containing 10% serum did not prevent the growth dependent decrease in glutathione. These results demonstrate that glutathione content in A549 cells is strongly affected by culture conditions.
研究了不同培养条件对A549（人II型肺肿瘤来源）细胞中谷胱甘肽含量的影响。所研究的参数包括生长时间、血清浓度以及胰岛素、转铁蛋白和亚硒酸混合物的有无。谷胱甘肽含量随血清浓度增加。当细胞在含血清的条件下生长时，传代后24小时谷胱甘肽急剧增加，此后下降。胰岛素、转铁蛋白和亚硒酸对细胞生长或谷胱甘肽含量影响很小。用含10%血清的新鲜培养基替换培养基并不能阻止谷胱甘肽随生长而减少。这些结果表明，A549细胞中的谷胱甘肽含量受培养条件的强烈影响。
Zotero 插件