Eady J J, Orta T, Dennis M F, Stratford M R, Peacock J H
Radiotherapy Research Unit, Institute of Cancer Research, Sutton, Surrey, UK.
Br J Cancer. 1995 Nov;72(5):1089-95. doi: 10.1038/bjc.1995.470.
Large fluctuations in glutathione content were observed on a daily basis using the Tietze enzyme recycling assay in a panel of six human cell lines of varying radiosensitivity. Glutathione content tended to increase to a maximum during exponential cell proliferation, and then decreased at different rates as the cells approached plateau phase. By reference to high-performance liquid chromatography and flow cytometry of the fluorescent bimane derivative we were able to verify that these changes were real. However, the Tietze assay was occasionally unable to detect glutathione in two of our cell lines (MGH-U1 and AT5BIVA), although the other methods indicated its presence. The existence of an inhibitory activity responsible for these anomalies was confirmed through spiking our samples with known amounts of glutathione. We were unable to detect a direct relationship between cellular glutathione concentration and aerobic radiosensitivity in our panel of cell lines.
使用蒂策酶循环测定法,在一组六种不同放射敏感性的人类细胞系中,每天都观察到谷胱甘肽含量的大幅波动。在指数细胞增殖期间,谷胱甘肽含量倾向于增加到最大值,然后随着细胞接近平台期以不同速率下降。通过参考荧光双硫腙衍生物的高效液相色谱法和流式细胞术,我们能够证实这些变化是真实的。然而,尽管其他方法表明谷胱甘肽存在,但蒂策测定法偶尔无法在我们的两种细胞系(MGH-U1和AT5BIVA)中检测到它。通过向我们的样品中加入已知量谷胱甘肽,证实了存在导致这些异常的抑制活性。在我们的细胞系组中,我们无法检测到细胞谷胱甘肽浓度与好氧放射敏感性之间的直接关系。