大肠杆菌中脂肪酸转运的调控：通过操纵子融合进行分析
Regulation of fatty acid transport in Escherichia coli: analysis by operon fusion.
作者信息
Sallus L, Haselbeck R J, Nunn W D
出版信息
J Bacteriol. 1983 Sep;155(3):1450-4. doi: 10.1128/jb.155.3.1450-1454.1983.
Abstract
The regulation of the fadL gene, which encodes a long-chain fatty acid (LCFA) transport component, was examined by constructing a strain of Escherichia coli K-12 that bears a phi (fadL-lacZ+) operon fusion plus a wild-type fadL gene. This merodiploid strain expressed LCFA transport and beta-galactosidase activity coordinately under noninducing, inducing, and catabolite-repressing conditions. Merodiploid strains which carried a defective fadR gene expressed LCFA transport and beta-galactosidase activity constitutively. These results suggest that expression of the fadL gene is regulated by the fadR gene and is inducible at the level of transcription by LCFA.
摘要
通过构建一株带有phi（fadL-lacZ+）操纵子融合体以及野生型fadL基因的大肠杆菌K-12菌株，研究了编码长链脂肪酸（LCFA）转运成分的fadL基因的调控情况。该部分二倍体菌株在非诱导、诱导和分解代谢物阻遏条件下，协调表达LCFA转运和β-半乳糖苷酶活性。携带缺陷型fadR基因的部分二倍体菌株组成型表达LCFA转运和β-半乳糖苷酶活性。这些结果表明，fadL基因的表达受fadR基因调控，并且在转录水平上可被LCFA诱导。
相似文献
1
Regulation of fatty acid transport in Escherichia coli: analysis by operon fusion.大肠杆菌中脂肪酸转运的调控：通过操纵子融合进行分析
J Bacteriol. 1983 Sep;155(3):1450-4. doi: 10.1128/jb.155.3.1450-1454.1983.
2
3
fadD deletion and fadL overexpression in Escherichia coli increase hydroxy long-chain fatty acid productivity.大肠杆菌中fadD基因缺失和fadL基因过表达可提高羟基长链脂肪酸的产量。
Appl Microbiol Biotechnol. 2014 Nov;98(21):8917-25. doi: 10.1007/s00253-014-5974-2. Epub 2014 Aug 14.
4
Transport of long-chain fatty acids by Escherichia coli: mapping and characterization of mutants in the fadL gene.大肠杆菌对长链脂肪酸的转运：fadL基因中突变体的定位与特性分析
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3377-81. doi: 10.1073/pnas.75.7.3377.
5
Regulation of transcription of genes required for fatty acid transport and unsaturated fatty acid biosynthesis in Escherichia coli by FadR.FadR对大肠杆菌中脂肪酸转运及不饱和脂肪酸生物合成所需基因转录的调控
Mol Microbiol. 1993 Jan;7(2):311-22. doi: 10.1111/j.1365-2958.1993.tb01122.x.
6
Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes.大肠杆菌 FadR 与全局调控因子在脂肪酸转运基因表达中的相互作用。
PLoS One. 2012;7(9):e46275. doi: 10.1371/journal.pone.0046275. Epub 2012 Sep 28.
7
Cloning and characterization of a gene (fadR) involved in regulation of fatty acid metabolism in Escherichia coli.大肠杆菌中参与脂肪酸代谢调控的一个基因（fadR）的克隆与特性分析
J Bacteriol. 1985 Feb;161(2):583-8. doi: 10.1128/jb.161.2.583-588.1985.
8
Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli.大肠杆菌中脂肪酸受体基因fadL的渗透调节
Mol Gen Genet. 1993 Sep;240(3):339-47. doi: 10.1007/BF00280384.
9
Regulation of fatty acid degradation in Escherichia coli: analysis by operon fusion.大肠杆菌中脂肪酸降解的调控：通过操纵子融合进行分析
J Bacteriol. 1981 Nov;148(2):521-6. doi: 10.1128/jb.148.2.521-526.1981.
10
Regulation of fatty acid degradation in Escherichia coli: dominance studies with strains merodiploid in gene fadR.大肠杆菌中脂肪酸降解的调控：fadR基因部分二倍体菌株的显性研究
J Bacteriol. 1980 Aug;143(2):726-30. doi: 10.1128/jb.143.2.726-730.1980.
引用本文的文献
1
Long-chain fatty acid transport in bacteria and yeast. Paradigms for defining the mechanism underlying this protein-mediated process.细菌和酵母中的长链脂肪酸转运。定义这种蛋白质介导过程潜在机制的范例。
Mol Cell Biochem. 1999 Feb;192(1-2):41-52.
2
Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli.大肠杆菌中脂肪酸受体基因fadL的渗透调节
Mol Gen Genet. 1993 Sep;240(3):339-47. doi: 10.1007/BF00280384.
3
Cloning and characterization of a gene (fadR) involved in regulation of fatty acid metabolism in Escherichia coli.大肠杆菌中参与脂肪酸代谢调控的一个基因（fadR）的克隆与特性分析
J Bacteriol. 1985 Feb;161(2):583-8. doi: 10.1128/jb.161.2.583-588.1985.
4
A molecular view of fatty acid catabolism in Escherichia coli.大肠杆菌中脂肪酸分解代谢的分子视角。
Microbiol Rev. 1986 Jun;50(2):179-92. doi: 10.1128/mr.50.2.179-192.1986.
5
The fadL gene product of Escherichia coli is an outer membrane protein required for uptake of long-chain fatty acids and involved in sensitivity to bacteriophage T2.大肠杆菌的fadL基因产物是一种外膜蛋白，是摄取长链脂肪酸所必需的，且与对噬菌体T2的敏感性有关。
J Bacteriol. 1988 Jun;170(6):2850-4. doi: 10.1128/jb.170.6.2850-2854.1988.
6
Uses of lac fusions for the study of biological problems.用于生物学问题研究的乳糖融合体的用途。
Microbiol Rev. 1985 Dec;49(4):398-418. doi: 10.1128/mr.49.4.398-418.1985.
7
Nucleotide sequence of the fadR gene, a multifunctional regulator of fatty acid metabolism in Escherichia coli.
Nucleic Acids Res. 1988 Aug 25;16(16):7995-8009. doi: 10.1093/nar/16.16.7995.
8
Primary sequence of the Escherichia coli fadL gene encoding an outer membrane protein required for long-chain fatty acid transport.编码长链脂肪酸转运所需外膜蛋白的大肠杆菌fadL基因的一级序列。
J Bacteriol. 1991 Jan;173(2):435-42. doi: 10.1128/jb.173.2.435-442.1991.
本文引用的文献
1
Regulation of fatty acid degradation in Escherichia coli: dominance studies with strains merodiploid in gene fadR.大肠杆菌中脂肪酸降解的调控：fadR基因部分二倍体菌株的显性研究
J Bacteriol. 1980 Aug;143(2):726-30. doi: 10.1128/jb.143.2.726-730.1980.
2
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
Regulation of fatty acid degradation in Escherichia coli: analysis by operon fusion.大肠杆菌中脂肪酸降解的调控：通过操纵子融合进行分析
J Bacteriol. 1981 Nov;148(2):521-6. doi: 10.1128/jb.148.2.521-526.1981.
4
Regulation of fatty acid degradation in Escherichia coli: isolation and characterization of strains bearing insertion and temperature-sensitive mutations in gene fadR.大肠杆菌中脂肪酸降解的调控：fadR基因中携带插入突变和温度敏感突变菌株的分离与鉴定
J Bacteriol. 1980 May;142(2):621-32. doi: 10.1128/jb.142.2.621-632.1980.
5
6
Control of fatty acid metabolism. I. Induction of the enzymes of fatty acid oxidation in Escherichia coli.脂肪酸代谢的调控。I. 大肠杆菌中脂肪酸氧化酶的诱导
J Bacteriol. 1969 Feb;97(2):827-36. doi: 10.1128/jb.97.2.827-836.1969.
7
The induction of the enzymes of fatty acid degradation in Escherichia coli.大肠杆菌中脂肪酸降解酶的诱导
Biochem Biophys Res Commun. 1967 Oct 11;29(1):28-33. doi: 10.1016/0006-291x(67)90535-9.
8
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.利用Mu-lac噬菌体一步法将乳糖基因与外源启动子融合：转录控制序列的体内探针
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3. doi: 10.1073/pnas.76.9.4530.
9
Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac gene fusions.大肠杆菌K-12中鞭毛蛋白基因（hag）表达的调控：hag-lac基因融合体分析
J Bacteriol. 1979 Sep;139(3):721-9. doi: 10.1128/jb.139.3.721-729.1979.
Zotero 插件