大肠杆菌K-12中鞭毛蛋白基因（hag）表达的调控：hag-lac基因融合体分析
Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac gene fusions.
作者信息
Komeda Y, Iino T
出版信息
J Bacteriol. 1979 Sep;139(3):721-9. doi: 10.1128/jb.139.3.721-729.1979.
Abstract
Previous studies have defined 28 genes necessary for the synthesis of the flagellar apparatus of Escherichia coli K-12. This study analyzed the influence of the flagellar genes on the expression of the hag gene (structural gene for flagellin). To this end, a hag::Mu d(Apr lac) mutant which had the lac genes fused to the promoter of the hag gene was constructed. This allowed the measurement of hag gene expression by detection of beta-galactosidase activity. The following observations were made. (i) The hag gene was expressed constitutively in Fla+ cells. (ii) hag gene expression was positively regulated by flaA, FLAB, flaC, flaD, flaE, flaG, flaH, flaI, flaK, flaL, flaM, flaN, flaO, flaP, flaQ, flaR, flaV, flaW, flaX, flaY, flaZ, flbA, and flbB genes.hag-lac expression was not observed in strains with these fla mutations. (iii) The hag gene was expressed in mutants with flaS, flaT, flaU, and flbC defects. Therefore, these genes were not involved in regulation of hag gene transcription.
摘要
先前的研究已经确定了大肠杆菌K-12鞭毛装置合成所必需的28个基因。本研究分析了鞭毛基因对hag基因（鞭毛蛋白的结构基因）表达的影响。为此，构建了一个hag::Mu d(Apr lac)突变体，该突变体的lac基因与hag基因的启动子融合。这使得通过检测β-半乳糖苷酶活性来测量hag基因的表达成为可能。得到了以下观察结果。（i）hag基因在Fla+细胞中组成性表达。（ii）hag基因的表达受到flaA、FLAB、flaC、flaD、flaE、flaG、flaH、flaI、flaK、flaL、flaM、flaN、flaO、flaP、flaQ、flaR、flaV、flaW、flaX、flaY、flaZ、flbA和flbB基因的正向调节。在具有这些fla突变的菌株中未观察到hag-lac表达。（iii）hag基因在具有flaS、flaT、flaU和flbC缺陷的突变体中表达。因此，这些基因不参与hag基因转录的调节。
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