利用Mu-lac噬菌体一步法将乳糖基因与外源启动子融合：转录控制序列的体内探针
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
作者信息
Casadaban M J, Cohen S N
出版信息
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3. doi: 10.1073/pnas.76.9.4530.
Abstract
The lactose structural genes, without the lactose promoter, have been incorporated into the bacteriophage Mu genome to form a Mu-lac specialized transducing phage. This phage also carries a gene encoding resistance to ampicillin (Ap)[Mu(Ap, lac)]. After infection and upon establishment of lysogeny, the Mu(Ap, lac) genome can integrate into apparently random sites in the Escherichia coli chromosome. When integration occurs within a gene in the orientation of its transcription, the lactose structural genes are so situated that they become expressed solely from the promoter of that gene. Thus, expression of the lactose genes of Mu(Ap, lac) can be used as an assay for transcription of that gene and for functional and mutational studies of gene regulation.
摘要
乳糖结构基因在没有乳糖启动子的情况下，已被整合到噬菌体Mu基因组中，形成了Mu-lac特异性转导噬菌体。这种噬菌体还携带一个编码对氨苄青霉素（Ap）抗性的基因[Mu（Ap，lac）]。感染后并在溶原状态建立时，Mu（Ap，lac）基因组可整合到大肠杆菌染色体中明显随机的位点。当整合发生在一个基因内且转录方向相同时，乳糖结构基因的位置使得它们仅从该基因的启动子开始表达。因此，Mu（Ap，lac）乳糖基因的表达可用于该基因转录的测定以及基因调控的功能和突变研究。
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