通过用固定红细胞吸收来消除甲状旁腺激素抗血清中对红细胞某些成分的交叉反应性。
Elimination of cross-reactivity to some component of erythrocytes in an antiserum to parathyroid hormone by absorption with fixed erythrocytes.
作者信息
Rao L G, Murray T M, Heersche J N
出版信息
J Histochem Cytochem. 1983 Sep;31(9):1163-5. doi: 10.1177/31.9.6350440.
PMID:6350440
Abstract
Cross-reactivity to some component of rat erythrocytes with an antiserum to parathyroid hormone (anti-PTH) was detected in fixed demineralized sections of bone using the peroxidase-antiperoxidase method. The cross-reactivity was eliminated by the preabsorption of anti-PTH with fixed, washed rat erythrocytes. This technique provides an easy and rapid method with which to eliminate cross-reactivity to erythrocytes whenever such a situation is encountered in immunohistochemical procedures.
摘要
采用过氧化物酶-抗过氧化物酶法在脱钙骨固定切片中检测到抗甲状旁腺激素血清（抗PTH）与大鼠红细胞某些成分的交叉反应性。用固定并洗涤过的大鼠红细胞预吸收抗PTH可消除交叉反应性。每当在免疫组织化学程序中遇到这种情况时，该技术提供了一种消除对红细胞交叉反应性的简便快速方法。
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Elimination of cross-reactivity to some component of erythrocytes in an antiserum to parathyroid hormone by absorption with fixed erythrocytes.通过用固定红细胞吸收来消除甲状旁腺激素抗血清中对红细胞某些成分的交叉反应性。
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