Engelman R W, Gengozian N, Good R A, Day N K
J Immunol Methods. 1985 Jul 16;81(1):65-71. doi: 10.1016/0022-1759(85)90122-x.
Optimal conditions of culture and assay for identification of feline immunoglobulin-secreting mononuclear cells were determined for the staphylococcal protein A-reverse hemolytic plaque assay (SpA-RHPA). Hemolytic plaques were most distinct and numerous when peripheral blood mononuclear cells were stimulated with 6.9 micrograms/ml pokeweed mitogen for 7 days. Immunoglobulin-secreting cells were identified morphologically within a zone of hemolysis utilizing a 1:5 dilution of rabbit anti-cat IgG and a 1:30 dilution of guinea pig complement as developing reagents. The SpA-RHPA system should contribute to an understanding of normal feline T- and B-lymphocyte interactions and will likely aid in the identification and understanding of immune cell dysfunctions associated with chronic feline leukemia virus infection.
针对葡萄球菌蛋白A反向溶血空斑试验（SpA-RHPA），确定了用于鉴定猫免疫球蛋白分泌单核细胞的最佳培养和检测条件。当外周血单核细胞用6.9微克/毫升商陆有丝分裂原刺激7天时，溶血空斑最为明显且数量最多。利用1:5稀释的兔抗猫IgG和1:30稀释的豚鼠补体作为显色试剂，在溶血区内通过形态学方法鉴定免疫球蛋白分泌细胞。SpA-RHPA系统应有助于理解正常猫的T淋巴细胞和B淋巴细胞相互作用，并且可能有助于鉴定和理解与猫慢性白血病病毒感染相关的免疫细胞功能障碍。
