Differential protein synthesis in Candida albicans during blastospore formation at 24.5 degrees C and during germ tube formation at 37 degrees C.

To identify proteins synthesized during blastospore to germ tube transformation in Candida albicans, membrane and cytoplasmic protein fractions labelled with 14C were analysed by SDS-PAGE and autoradiography. Four cytoplasmic proteins were detected in pulse-labelled lysates prepared from cells forming blastospores and germ tubes at 37 degrees C, but not in pulse-labelled lysates prepared from cells forming blastospores at 24.5 degrees C. Three proteins detectable in 24.5 degrees C lysates were diminished in 37 degrees C lysates.