Koltai M, Blazsó G, Minker E
Agents Actions. 1983 Jun;13(4):283-7. doi: 10.1007/BF01971479.
The effect of supernatants of isolated lymph node cells exposed to insulin has been studied on the anaphylactoid reaction induced by dextran in the rat paw. Native supernatants derived from rat lymphocytes were shown to increase dextran response only from April to October, while in the intermediate period no potentiation was obtained. The supernatants were filtered through sephadex G-25, G-50, and G-100 gels. Pro-inflammatory activity was recovered in the void volume of sephadex G-25 or G-50 columns, while it ran with bovine serum albumin marker suggesting an approximate mol. wt. of 70-kilodalton. Potentiation was detectable in adrenalectomized rats from November through April. A fraction of the eluate recovered from sephadex G-25 gels in the range of 1- to 6-kilodalton was found to suppress dextran edema. Supernatants of calf lymph node cells with insulin were fractionated on DEAE-sephadex A-50 and yielded two distinct fractions, one with pro-inflammatory and the other with anti-inflammatory effect. These results suggest that insulin affects anaphylactoid reaction via the release of some lymphocyte mediators.
研究了暴露于胰岛素的分离淋巴结细胞的上清液对右旋糖酐诱导的大鼠爪类过敏反应的影响。来自大鼠淋巴细胞的天然上清液仅在4月至10月显示出增强右旋糖酐反应，而在中间时期未获得增强作用。将上清液通过葡聚糖凝胶G-25、G-50和G-100过滤。促炎活性在葡聚糖凝胶G-25或G-50柱的空体积中恢复，而它与牛血清白蛋白标记物一起移动，表明分子量约为70千道尔顿。从11月到4月，在肾上腺切除的大鼠中可检测到增强作用。发现从葡聚糖凝胶G-25凝胶中回收的1至6千道尔顿范围内的洗脱液部分可抑制右旋糖酐水肿。用胰岛素处理的小牛淋巴结细胞的上清液在DEAE-葡聚糖凝胶A-50上进行分级分离，得到两个不同的级分，一个具有促炎作用，另一个具有抗炎作用。这些结果表明，胰岛素通过释放一些淋巴细胞介质影响类过敏反应。
