Phizackerley P J, Al-Dabbagh S A
Anal Biochem. 1983 May;131(1):242-5. doi: 10.1016/0003-2697(83)90161-6.
A method for the estimation of nitrate and nitrite is described in which nitrate is converted to nitrite by Klebsiella pneumoniae (UNF 9232) and nitrite is estimated by the Griess reaction before and after incubation. The method is suitable for the estimation of 1-25 nmol of each ion in body fluids, many samples can be handled simultaneously, and special apparatus is not required.
本文描述了一种硝酸盐和亚硝酸盐的估算方法,其中硝酸盐通过肺炎克雷伯菌(UNF 9232)转化为亚硝酸盐,亚硝酸盐在孵育前后通过格里斯反应进行估算。该方法适用于估算体液中每种离子1-25纳摩尔的含量,可同时处理多个样本,且无需特殊仪器。