Larsson U K, Jergil B, Andersson B
Eur J Biochem. 1983 Oct 17;136(1):25-9. doi: 10.1111/j.1432-1033.1983.tb07700.x.
The effect of protein phosphorylation on the distribution of chlorophyll-protein complexes between appressed and non-appressed thylakoid regions of spinach chloroplasts has been investigated. Stroma lamellae vesicles and inside-out vesicles, representative of non-appressed and appressed thylakoids, respectively, were isolated from thylakoid membranes before and after phosphorylation. The fractions were analyzed with respect to the yield of vesicles, incorporation of [32P]phosphate into the light-harvesting chlorophyll-a/b--protein complex, chlorophyll composition, and the relative content of the main chlorophyll-protein complexes. The yield of inside-out vesicles from phosphorylated thylakoids was 20% lower than that from control thylakoids, indicating a partial destacking. The specific incorporation of [32P]phosphate into the light-harvesting chlorophyll-a/b--protein complex was at least four times higher in stroma lamellae vesicles than in inside-out vesicles. The proportion of the light-harvesting chlorophyll-a/b--protein complex in stroma lamellae vesicles increased from 13% to 21% of their total chlorophyll after phosphorylation of the thylakoids. There was also a corresponding increase in the level of chlorophyll b. These observations strongly suggest that phosphorylated light-harvesting complexes migrate from appressed thylakoids rich in photosystem 2 to non-appressed thylakoids rich in photosystem 1. In contrast, there was no evidence for a lateral migration of the chlorophyll-a--protein complex of photosystem 2 after phosphorylation. Our results indicate that a lateral migration of phosphorylated light-harvesting complexes in combination with a lateral separation of photosystem 1 and 2 to the different thylakoid regions, is a basis for the regulation of excitation energy between the two photosystems.
研究了蛋白质磷酸化对菠菜叶绿体中叶绿素 - 蛋白质复合物在垛叠和非垛叠类囊体区域之间分布的影响。分别代表非垛叠和垛叠类囊体的基质片层囊泡和内翻囊泡，在磷酸化前后从类囊体膜中分离出来。对这些组分进行了囊泡产量、[32P]磷酸盐掺入捕光叶绿素 - a/b - 蛋白质复合物、叶绿素组成以及主要叶绿素 - 蛋白质复合物相对含量的分析。磷酸化类囊体产生的内翻囊泡产量比对照类囊体低20%，表明部分解垛叠。基质片层囊泡中[32P]磷酸盐掺入捕光叶绿素 - a/b - 蛋白质复合物的特异性至少是内翻囊泡中的四倍。类囊体磷酸化后，基质片层囊泡中捕光叶绿素 - a/b - 蛋白质复合物占其总叶绿素的比例从13%增加到21%。叶绿素b的水平也相应增加。这些观察结果有力地表明，磷酸化的捕光复合物从富含光系统2的垛叠类囊体迁移到富含光系统1的非垛叠类囊体。相反，没有证据表明磷酸化后光系统2的叶绿素 - a - 蛋白质复合物发生横向迁移。我们的结果表明，磷酸化的捕光复合物的横向迁移与光系统1和2在不同类囊体区域的横向分离相结合，是两个光系统之间激发能调节的基础。
