印度麂中期染色体中富含5-甲基胞嘧啶区域的免疫可视化
Immunological visualization of 5-methylcytosine-rich regions in Indian Muntjac metaphase chromosomes.
作者信息
Vasilikaki-Baker H, Nishioka Y
出版信息
Exp Cell Res. 1983 Aug;147(1):226-30. doi: 10.1016/0014-4827(83)90289-6.
Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antibody (anti 5-MeC), regions of antibody binding were visualized by an immunofluorescence or immunopreoxidase staining procedure. All chromosomes showed some level of antibody binding along their length and at centromeric regions, with intense binding evident in the centromere of chromosome 3 and the elongated centromeric "neck" of chromosome 3-X. The Y chromosome displayed low levels of antibody binding. The banding pattern observed with anti 5-MeC is the reverse of that obtained by quinacrine staining.
富含5-甲基胞嘧啶的区域定位于印度麂（Muntiakus muntjak）雄性中期染色体上。对染色体进行紫外线照射，随后在亚甲蓝存在的情况下进行光氧化，以诱导最大程度的DNA变性。用抗5-甲基胞嘧啶抗体（抗5-MeC）处理后，通过免疫荧光或免疫过氧化物酶染色程序观察抗体结合区域。所有染色体在其全长以及着丝粒区域均显示出一定程度的抗体结合，在3号染色体的着丝粒以及3-X染色体拉长的着丝粒“颈部”可见强烈结合。Y染色体显示出低水平的抗体结合。抗5-MeC观察到的带型与喹吖因染色得到的带型相反。
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