Schnedl W, Dev V G, Tantravahi R, Miller D A, Erlanger B F, Miller O J
Chromosoma. 1975 Sep 15;52(1):59-66. doi: 10.1007/BF00285789.
Fixed metaphase chromosomes of gorilla and chimpanzee were UV-irradiated to produce regions of single-stranded DNA and then treated with antibodies specific for the minor DNA base 5-methylcytosine (5 MeC). An indirect immunofluorescence technique was used to visualize sites of antibody binding. In the gorilla six pairs of autosomes contained major fluorescent regions, indicating localized regions of highly methylated DNA. These corresponded, with the exception of chromosome 19, to the major regions of constitutive heterochromatin as seen by C-banding. The Y chromosome also contained a highly fluorescent region which was located just proximal to the intense Q-band region. In the chimpanzee no comparable concentrations of highly methylated DNA were seen. Smaller regions of intense 5 MeC binding were present on perhaps six chimpanzee chromosomes, including the Y. Five of these corresponded to chromosomes which were highly methylated in the gorilla.--There is diversity among the human, gorilla and chimpanzee in both the size and location of concentrations of 5 MeC, supporting the idea that satellite DNA evolves more rapidly than DNA in the remainder of the chromosome.
对大猩猩和黑猩猩的固定中期染色体进行紫外线照射，以产生单链DNA区域，然后用针对次要DNA碱基5-甲基胞嘧啶（5MeC）的特异性抗体进行处理。采用间接免疫荧光技术来观察抗体结合位点。在大猩猩中，六对常染色体含有主要荧光区域，表明存在高度甲基化DNA的局部区域。除了19号染色体外，这些区域与C带显示的组成型异染色质主要区域相对应。Y染色体也含有一个高度荧光区域，位于强烈Q带区域的近端。在黑猩猩中，未观察到高度甲基化DNA的类似集中情况。在包括Y染色体在内的大约六条黑猩猩染色体上存在较小的强烈5MeC结合区域。其中五条对应于在大猩猩中高度甲基化的染色体。——人类、大猩猩和黑猩猩在5MeC浓度的大小和位置上存在差异，这支持了卫星DNA比染色体其余部分的DNA进化更快的观点。
