Klein J R, Monjan A A
Immunol Commun. 1983;12(1):25-30. doi: 10.3109/08820138309060854.
C3H/HeJ mice which had been primed with either virulent or killed T. pallidum were studied for in vivo delayed-type hypersensitivity (DTH) responses to T. pallidum following local footpad challenge. Mice sustaining a chronic infection of 5 months duration failed to develop a DTH to treponemal antigens, whereas priming by a single intravenous injection with killed organisms resulted in a significant DTH response in mice when challenged 5 days later. Treatment of mice prior to priming with a single sublethal dose of cyclophosphamide (100 mg/kg body weight) not only failed to potentiate T. pallidum-DTH, but abrogated the response observed in untreated primed animals.
用强毒或灭活梅毒螺旋体致敏的C3H/HeJ小鼠，在局部足垫激发后，研究其对梅毒螺旋体的体内迟发型超敏反应（DTH）。持续感染5个月的慢性感染小鼠未能产生针对梅毒螺旋体抗原的DTH，而单次静脉注射灭活菌致敏的小鼠在5天后受到激发时则产生了显著的DTH反应。在致敏前用单剂量亚致死量环磷酰胺（100 mg/kg体重）处理小鼠，不仅未能增强梅毒螺旋体-DTH反应，反而消除了未处理致敏动物中观察到的反应。
