Richmond J E
Int J Tissue React. 1983;5(2):159-63.
Rotational-mediated aggregation techniques of 7-day chick embryo lung cells were performed in the presence of 1-3H-glucosamine and 1-14C-glycine, and hormones were added to assess some of the factors involved in the formation of histotypic structures and organogenesis. 3,3',5-Tri-iodo-thyronine, 10(-8)M, stimulated 1-3H-glucosamine incorporation 300%, while insulin, 10(-6)M, led to a 70% augmentation and the combination of 10(-8)M 3,3',5-tri-iodo-thyronine and 10(-6)M insulin resulted in a 400% increase in 1-3-H-glucosamine incorporation. Glycine-1-14C incorporation was enhanced 70% by tri-iodo-thyronine and 60% by insulin. Tri-iodo-thyronine and insulin produced a 200% increase in 14C labelling from 1-14C-glycine. The effects of the combination of these two hormones appear to be additive; insulin stimulation of aggregation or the formation of histotypic structures paralleled the stimulation of the incorporation of glucosamine and glycine.
在含有(1-^3H -)葡萄糖胺和(1-^{14}C -)甘氨酸的条件下，对7日龄鸡胚肺细胞进行旋转介导的聚集技术操作，并添加激素以评估参与组织型结构形成和器官发生的一些因素。(10^{-8}M)的三碘甲状腺原氨酸使(1-^3H -)葡萄糖胺掺入增加(300%)，而(10^{-6}M)的胰岛素使掺入增加(70%)，(10^{-8}M)的三碘甲状腺原氨酸与(10^{-6}M)的胰岛素联合使用使(1-^3H -)葡萄糖胺掺入增加(400%)。三碘甲状腺原氨酸使甘氨酸(-1-^{14}C)掺入增加(70%)，胰岛素使其增加(60%)。三碘甲状腺原氨酸和胰岛素使(1-^{14}C -)甘氨酸的(^{14}C)标记增加(200%)。这两种激素联合使用的效果似乎是相加的；胰岛素对聚集或组织型结构形成的刺激与对葡萄糖胺和甘氨酸掺入的刺激平行。
