Richmond J E
Int J Tissue React. 1983;5(2):153-8.
Seven-day chick embryo lung was dissociated into single cells and the capacity of these cells to incorporate 1-3H-glucosamine and 1-14C-glycine in a rotational-mediated aggregation system upon the addition of hormones was determined. Incorporation of 1-3H-glucosamine was stimulated 50% by 10(-8) M dexamethasone while 1-14C glycine uptake was not markedly influenced. Dexamethasone, 10(-8) M and 10(-6) M insulin gave rise to a 90% enhancement in 1-3H-glucosamine incorporation and a 20% increase in 14C-glycine uptake. When the aggregating cells were incubated with 10(-8) M dexamethasone and 10(-8) M 3,3',5-tri-iodo-thyronine, glucosamine incorporation was augmented by 40% and glycine by 25%. The combination, 10(-8) M 3,3',5-tri-iodo-thyronine, 10(-8) M dexamethasone and 10(-6) M insulin led to a 70% stimulation in glucosamine labelling and a 50% increase in 1-14C-glycine uptake. Very little change in the incorporation of the hexosamine and glycine into the soluble culture media was observed by adding either of the hormones; however, there appeared to be a slight increase in the incorporation of both tracers when the cells were incubated with the combination of dexamethasone and insulin.
将7日龄鸡胚肺组织解离成单细胞，测定这些细胞在添加激素后，于旋转介导聚集系统中掺入³H-葡糖胺和¹⁴C-甘氨酸的能力。10⁻⁸M地塞米松可使³H-葡糖胺的掺入量增加50%，而¹⁴C-甘氨酸的摄取量未受到明显影响。10⁻⁸M和10⁻⁶M胰岛素可使³H-葡糖胺的掺入量提高90%，¹⁴C-甘氨酸的摄取量增加20%。当聚集细胞与10⁻⁸M地塞米松和10⁻⁸M三碘甲腺原氨酸一起孵育时，葡糖胺的掺入量增加40%，甘氨酸增加25%。10⁻⁸M三碘甲腺原氨酸、10⁻⁸M地塞米松和10⁻⁶M胰岛素的组合可使葡糖胺标记增加70%，¹⁴C-甘氨酸的摄取量增加50%。添加任何一种激素后，己糖胺和甘氨酸掺入可溶性培养基中的变化甚微；然而，当细胞与地塞米松和胰岛素组合孵育时，两种示踪剂的掺入量似乎略有增加。
