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Purification of recA protein from Escherichia coli.

作者信息

Shibata T, Osber L, Radding C M

出版信息

Methods Enzymol. 1983;100:197-209. doi: 10.1016/0076-6879(83)00056-7.

DOI:10.1016/0076-6879(83)00056-7
PMID:6353142
Abstract
摘要

相似文献

1
Purification of recA protein from Escherichia coli.从大肠杆菌中纯化重组A蛋白。
Methods Enzymol. 1983;100:197-209. doi: 10.1016/0076-6879(83)00056-7.
2
Intein-mediated affinity-fusion purification of the Escherichia coli RecA protein.
Protein Expr Purif. 2002 Dec;26(3):476-88. doi: 10.1016/s1046-5928(02)00571-5.
3
Purification of recA-based fusion proteins by immunoadsorbent chromatography. Characterization of a major antigenic determinant of Escherichia coli recA protein.
J Biol Chem. 1985 Aug 25;260(18):10263-7.
4
RecA protein rapidly crystallizes in the presence of spermidine: a valuable step in its purification and physical characterization.在亚精胺存在的情况下,RecA蛋白迅速结晶:这是其纯化和物理特性表征中的重要一步。
Biochemistry. 1985 Jan 1;24(1):158-62. doi: 10.1021/bi00322a022.
5
Induction of recombination by the bacterial RecA protein depends on the stability of the RecA-DNA complex.
Dokl Biochem. 2000 Mar-Apr;371(1-6):43-5.
6
A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli.一种用于大规模纯化大肠杆菌RecA蛋白的简单快速方法。
J Biol Chem. 1981 May 10;256(9):4676-8.
7
Genetic recombination in Escherichia coli: Holliday junctions made by RecA protein are resolved by fractionated cell-free extracts.大肠杆菌中的基因重组:RecA 蛋白形成的霍利迪连接体由分级分离的无细胞提取物解析。
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8476-80. doi: 10.1073/pnas.87.21.8476.
8
Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins.大肠杆菌RecA和RuvC蛋白对重组中间体的形成与解析
Nature. 1991;354(6354):506-10. doi: 10.1038/354506a0.
9
Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene.通过克隆的purA基因抑制大肠杆菌K-12的rdgB突变体中的缺陷。
J Bacteriol. 1991 Feb;173(3):1360-2. doi: 10.1128/jb.173.3.1360-1362.1991.
10
Cloning, characterization and expression of the recA gene of Aeromonas salmonicida.
Gene. 1996 Oct 10;175(1-2):133-6. doi: 10.1016/0378-1119(96)00138-2.

引用本文的文献

1
Loop L1 governs the DNA-binding specificity and order for RecA-catalyzed reactions in homologous recombination and DNA repair.环L1在同源重组和DNA修复中决定RecA催化反应的DNA结合特异性和顺序。
Nucleic Acids Res. 2015 Jan;43(2):973-86. doi: 10.1093/nar/gku1364. Epub 2015 Jan 5.
2
An NMR study on the interaction of Escherichia coli DinI with RecA-ssDNA complexes.一项关于大肠杆菌DinI与RecA-ssDNA复合物相互作用的核磁共振研究。
Nucleic Acids Res. 2003 Mar 15;31(6):1735-43. doi: 10.1093/nar/gkg256.
3
Yeast cell-free system that catalyses joint-molecule formation in a Rad51p- and Rad52p-dependent fashion.
酵母无细胞系统,其以依赖Rad51p和Rad52p的方式催化联合分子形成。
Biochem J. 2000 Apr 15;347(Pt 2):363-8. doi: 10.1042/0264-6021:3470363.
4
Helical repeat of DNA in the region of homologous pairing.同源配对区域中DNA的螺旋重复。
Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7837-40. doi: 10.1073/pnas.94.15.7837.
5
An extended DNA structure through deoxyribose-base stacking induced by RecA protein.由RecA蛋白诱导的通过脱氧核糖-碱基堆积形成的延伸DNA结构。
Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6623-8. doi: 10.1073/pnas.94.13.6623.
6
Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein.大肠杆菌RecF、RecO和RecR蛋白与RecA蛋白及单链DNA结合蛋白的生化相互作用。
Proc Natl Acad Sci U S A. 1993 May 1;90(9):3875-9. doi: 10.1073/pnas.90.9.3875.
7
A chimeric Rec-A protein that implicates non-Watson-Crick interactions in homologous pairing.一种嵌合Rec-A蛋白,其在同源配对中涉及非沃森-克里克相互作用。
Nucleic Acids Res. 1994 Aug 25;22(16):3387-91. doi: 10.1093/nar/22.16.3387.
8
Effects of various single-stranded-DNA-binding proteins on reactions promoted by RecA protein.各种单链DNA结合蛋白对RecA蛋白所促进反应的影响。
J Bacteriol. 1987 Aug;169(8):3422-8. doi: 10.1128/jb.169.8.3422-3428.1987.
9
Phenol-treatment and a homologous pairing-assay.苯酚处理和同源配对分析。
Nucleic Acids Res. 1992 Jul 25;20(14):3679-84. doi: 10.1093/nar/20.14.3679.
10
The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase.纯化的酵母前体mRNA剪接因子PRP2是一种依赖RNA的NTP酶。
EMBO J. 1992 Jun;11(6):2319-26. doi: 10.1002/j.1460-2075.1992.tb05291.x.