Waterman M R, Estabrook R W
Mol Cell Biochem. 1983;53-54(1-2):267-78. doi: 10.1007/BF00225259.
Liver endoplasmic reticulum contains as NADPH-dependent electron transport complex where the family of hemeproteins, termed cytochrome P-450, serve as catalysts for the oxidation of a variety of different organic chemicals. The content and inventory of the types of cytochrome P-450 is readily modified following in vivo treatment of animals with 'inducing agents' such as barbiturates, steroids and polycyclic hydrocarbons. Recent studies have applied the methods of molecular biology to evaluate changes in the transcription and translation of genomic information occurring concomitant with the initiation of synthesis of various types of cytochrome P-450. The ability to isolate unique cytochrome P-450 proteins and to prepare specific antibodies now permits the study of in vitro translation of mRNA and the preparation of specific cDNAs. The present review summarizes the historic background leading to current concepts of cytochrome P-450 induction and describes recent advances in our knowledge of the regulation of cytochrome P-450 synthesis in the liver.
肝脏内质网含有一种作为NADPH依赖电子传递复合物的物质，其中被称为细胞色素P - 450的血红素蛋白家族，作为多种不同有机化学物质氧化反应的催化剂。在用巴比妥酸盐、类固醇和多环烃等“诱导剂”对动物进行体内处理后，细胞色素P - 450类型的含量和存量很容易发生改变。最近的研究应用分子生物学方法来评估与各种类型细胞色素P - 450合成起始同时发生的基因组信息转录和翻译的变化。现在，分离独特细胞色素P - 450蛋白和制备特异性抗体的能力使得对mRNA的体外翻译研究以及特异性cDNA的制备成为可能。本综述总结了导致细胞色素P - 450诱导当前概念的历史背景，并描述了我们在肝脏中细胞色素P - 450合成调控知识方面的最新进展。
