Haffmans J, Blankwater Y J, Ukponmwan O E, Zijlstra F J, Vincent J E, Hespe W, Dzoljic M R
Neuropharmacology. 1983 Aug;22(8):1021-8. doi: 10.1016/0028-3908(83)90219-8.
The correlation between the distribution of the intraventricularly (i.v.t.) administered delta agonist 3H-enkephalin ([3H]DADL) and the anatomical regions involved in enkephalin-induced seizures has been studied in rat by using an autoradiographic method and recording of the electromyogram (EMG) and the electroencephalogram (EEG). The results indicate that within 10 min, the radioactivity of the intraventricularly administered drug reached all parts of the ventricular system, including the central canal of the spinal cord. However, within 2.5 min after the intraventricular administration of [3H]DADL, which corresponds to the onset of DADL-induced seizures, the substance appeared mainly in the left lateral ventricle and occasionally in the third ventricle. During the first 2.5 min the substance penetrated regularly into the surrounding periventricular tissue of the striatum, septum and hippocampus to a depth of about 100 microns. The most intensive and long-lasting epileptic discharges, exceeding 30 min were observed in the hippocampus, in contrast to the mild and short-lasting electrophysiological responses of the septum and corpus striatum. The experiments suggest that the short onset of enkephalin-induced excitatory phenomena is due to the rapid distribution and penetration of the substance in the surrounding periventricular tissue. According to these data, it is proposed that activation of delta opiate receptors, localized within the first 100 microns of the periventricular tissue, mainly in the hippocampus, is essential for the triggering of endorphin-induced seizure activity.
采用放射自显影法并记录肌电图（EMG）和脑电图（EEG），研究了脑室注射δ激动剂³H-脑啡肽([³H]DADL)的分布与参与脑啡肽诱发癫痫发作的解剖区域之间的相关性。结果表明，在10分钟内，脑室内注射药物的放射性到达脑室系统的所有部位，包括脊髓中央管。然而，在脑室内注射[³H]DADL后2.5分钟内，即对应于DADL诱发癫痫发作的开始，该物质主要出现在左侧脑室，偶尔出现在第三脑室。在最初的2.5分钟内，该物质有规律地渗透到纹状体、隔区和海马周围的脑室周围组织，深度约为100微米。与隔区和纹状体轻微且短暂的电生理反应相比，在海马中观察到最强烈且持续时间最长的癫痫放电，超过30分钟。实验表明，脑啡肽诱发的兴奋现象起效迅速是由于该物质在周围脑室周围组织中的快速分布和渗透。根据这些数据，有人提出，位于脑室周围组织最初100微米内、主要在海马中的δ阿片受体的激活对于触发内啡肽诱发的癫痫活动至关重要。
