Taylor I L, Vaillant C R
Peptides. 1983 Mar-Apr;4(2):245-53. doi: 10.1016/0196-9781(83)90122-5.
A region-specific antiserum (AbS11) raised against the carboxyl-terminal hexapeptide of pancreatic polypeptide has been employed to measure rat pancreatic polypeptide specifically and to demonstrate apparent immunoreactivity in nerves and in endocrine cells outside the pancreas. The concentration of pancreatic polypeptide in the head of the rat pancreas measured with AbS11 (176 +/- 47 pmol/g) was 750 fold higher than that measured with a conventional anti-bPP antiserum (0.23 +/- 0.08 pmol/g). Column chromatographs of rat pancreatic extracts demonstrated two peaks of immunoreactivity both eluting after the porcine pancreatic polypeptide standard. AbS11 also detected specific immunoreactivity in rat brain (470 fmol/g) which went undetected in convention assays. Although immunohistochemical studies with AbS11 and human pancreatic polypeptide antiserum demonstrated immunoreactivity in the same population of pancreatic endocrine cells, immunoreactive nerve fibres and enteroglucagon cells were only demonstrable with AbS11. These studies demonstrate that the carboxyl terminus of rat pancreatic polypeptide is immunochemically similar to that of higher mammals. Furthermore, neural and extrapancreatic endocrine variants of this peptide share an immunochemical determinant contained within the carboxyl-terminal hexapeptide.
一种针对胰多肽羧基末端六肽产生的区域特异性抗血清（AbS11）已被用于特异性测量大鼠胰多肽，并在神经和胰腺外的内分泌细胞中显示出明显的免疫反应性。用AbS11测量的大鼠胰头中胰多肽浓度（176±47 pmol/g）比用传统抗bPP抗血清测量的浓度（0.23±0.08 pmol/g）高750倍。大鼠胰腺提取物的柱色谱显示出两个免疫反应性峰，均在猪胰多肽标准品之后洗脱。AbS11还在大鼠脑中检测到特异性免疫反应性（470 fmol/g），而在传统检测中未检测到。尽管用AbS11和人胰多肽抗血清进行的免疫组织化学研究显示在同一群胰腺内分泌细胞中有免疫反应性，但免疫反应性神经纤维和肠高血糖素细胞仅用AbS11可显示。这些研究表明，大鼠胰多肽的羧基末端在免疫化学上与高等哺乳动物的相似。此外，该肽的神经和胰腺外内分泌变体共享羧基末端六肽中包含的免疫化学决定簇。
