Vaillant C, Taylor I L
Peptides. 1981;2 Suppl 2:31-5. doi: 10.1016/0196-9781(81)90006-1.
Pancreatic polypeptide (PP) has been isolated from the pancreas of several species, and has been localized by immunohistochemistry to a distinct population of pancreatic endocrine cells. Recently, PP-like immunoreactivity has been demonstrated outside the pancreas, in gut endocrine cells and central and peripheral nerves, in studies using antisera raised to natural PPs. The antigenic determinants recognised by these antisera are not known in any detail, and, therefore, the identity of the molecules in these extrapancreatic localizations is also unknown. We have raised an antiserum to the synthetic COOH-terminus of PP (PP6), which, therefore, is directed exclusively towards the biologically active, invariant region of mammalian PPs. PP6-like immunoreactivity was localized in pancreatic cells which were revealed also by antiserum to human PP (hPP). Outside the pancreas, no localizations were obtained with anti-hPP antiserum, whereas antiserum to PP6 demonstrated immunoreactivity in a variety of tissues including gut glucagon cells, adrenal chromaffin cells, and nerve fibres in the gastrointestinal tract and adrenal gland. We would suggest that the wide distribution in nerves and endocrine cells of PP6-immunoreactive material, which may be biologically active, should be taken into account in the search for a physiological role for PP.
胰多肽（PP）已从多种动物的胰腺中分离出来，并通过免疫组织化学定位到胰腺内分泌细胞的一个独特群体。最近，在使用针对天然PPs产生的抗血清的研究中，已证明在胰腺外、肠道内分泌细胞以及中枢和外周神经中存在PP样免疫反应性。这些抗血清识别的抗原决定簇尚不清楚，因此，这些胰腺外定位中分子的身份也未知。我们制备了一种针对PP合成COOH末端（PP6）的抗血清，因此，它仅针对哺乳动物PPs的生物活性不变区域。PP6样免疫反应性定位于胰腺细胞，抗人PP（hPP）抗血清也显示了这些细胞。在胰腺外，抗hPP抗血清未获得定位结果，而抗PP6抗血清在多种组织中显示出免疫反应性，包括肠道胰高血糖素细胞、肾上腺嗜铬细胞以及胃肠道和肾上腺中的神经纤维。我们认为，在寻找PP的生理作用时，应考虑PP6免疫反应性物质在神经和内分泌细胞中的广泛分布，这种物质可能具有生物活性。
