Pandey S K, Chiasson J L, Srivastava A K
Centre de Recherche/Hotel-Dieu de Montreal Hospital, Quebec, Canada.
Mol Cell Biochem. 1995;153(1-2):69-78. doi: 10.1007/BF01075920.
Effect of several vanadium salts, sodium orthovanadate, vanadyl sulfate and sodium metavanadate on protein tyrosine phosphorylation and serine/threonine kinases in chinese hamster ovary (CHO) cells overexpressing a normal human insulin receptor was examined. All the compounds stimulated protein tyrosine phosphorylation of two major proteins with molecular masses of 42 kDa (p42) and 44 kDa (p44). The phosphorylation of p42 and p44 was associated with an activation of mitogen activated protein (MAP) kinase as well as increased protein tyrosine phosphorylation of p42mapk and p44mapk. Vanadium salts also activated the 90 kDa ribosomal s6 kinase (p90rsk) and 70 kDa ribosomal s6 kinase (p70s6k). Among the three vanadium salts tested, vanadyl sulfate appeared to be slightly more potent than others in stimulating MAP kinases and p70s6k activity. It is suggested that vanadium-induced activation of MAP kinases and ribosomal s6 kinases may be one of the mechanisms by which insulin like effects of this trace element are mediated.
研究了几种钒盐（原钒酸钠、硫酸氧钒和偏钒酸钠）对过表达正常人胰岛素受体的中国仓鼠卵巢（CHO）细胞中蛋白质酪氨酸磷酸化和丝氨酸/苏氨酸激酶的影响。所有化合物均刺激了两种主要蛋白质的蛋白质酪氨酸磷酸化，这两种蛋白质的分子量分别为42 kDa（p42）和44 kDa（p44）。p42和p44的磷酸化与丝裂原活化蛋白（MAP）激酶的激活以及p42mapk和p44mapk的蛋白质酪氨酸磷酸化增加有关。钒盐还激活了90 kDa核糖体s6激酶（p90rsk）和70 kDa核糖体s6激酶（p70s6k）。在所测试的三种钒盐中，硫酸氧钒在刺激MAP激酶和p70s6k活性方面似乎比其他钒盐稍强。有人提出，钒诱导的MAP激酶和核糖体s6激酶的激活可能是该微量元素的胰岛素样作用介导的机制之一。
