Bagnasco M, Ferrini S, Merli A, Dirienzo W, Ciprandi G, Melioli G
Ric Clin Lab. 1983 Apr-Jun;13(2):247-54. doi: 10.1007/BF02904838.
A rapid and simple enzyme-linked immunosorbent assay (ELISA) for anti-thyroglobulin IgG antibodies is described. The method is based on a 'sandwich' using purified human thyroglobulin adsorbed to polystyrene microplates, human serum and anti-human IgG antiserum conjugated to alkaline phosphatase. The sensitivity of the method is about 8 ng/ml, as evaluated with a purified anti-thyroglobulin antibody preparation. High concentrations of antibodies were observed, as expected, in autoimmune thyroid disease; however, the majority of normal subjects have detectable, although very low, antibody levels. We conclude the method is suitable for current clinical use.
本文描述了一种用于检测抗甲状腺球蛋白IgG抗体的快速简便的酶联免疫吸附测定（ELISA）方法。该方法基于一种“夹心”技术，使用吸附于聚苯乙烯微孔板上的纯化人甲状腺球蛋白、人血清以及与碱性磷酸酶偶联的抗人IgG抗血清。用纯化的抗甲状腺球蛋白抗体制剂评估，该方法的灵敏度约为8 ng/ml。正如预期的那样，在自身免疫性甲状腺疾病中观察到高浓度的抗体；然而，大多数正常受试者也有可检测到的抗体水平，尽管非常低。我们得出结论，该方法适用于当前的临床应用。
