恶臭假单胞菌NCIB9816编码萘双加氧酶基因的克隆、核苷酸序列及特性分析
Cloning, nucleotide sequence and characterization of genes encoding naphthalene dioxygenase of Pseudomonas putida strain NCIB9816.
作者信息
Kurkela S, Lehväslaiho H, Palva E T, Teeri T H
机构信息
Department of Genetics, University of Helsinki, Finland.
出版信息
Gene. 1988 Dec 20;73(2):355-62. doi: 10.1016/0378-1119(88)90500-8.
We have cloned the naphthalene dioxygenase(ND)-coding genes from Pseudomonas putida strain NCIB9816 based on their ability to convert indole to indigo. The region coding for this enzyme activity was sequenced and three successive open reading frames were found. The corresponding gene products were identified using the T7 polymerase/promoter system. All of them are necessary for the ND activity. A comparison of the ND-coding genes with the ones coding for benzene dioxygenase revealed significant homology which was more pronounced at the nucleotide level than at the amino acid level.
我们基于恶臭假单胞菌菌株NCIB9816将吲哚转化为靛蓝的能力，克隆了萘双加氧酶（ND）编码基因。对编码该酶活性的区域进行了测序，发现了三个连续的开放阅读框。使用T7聚合酶/启动子系统鉴定了相应的基因产物。它们都是ND活性所必需的。将ND编码基因与编码苯双加氧酶的基因进行比较，发现了显著的同源性，这种同源性在核苷酸水平上比在氨基酸水平上更为明显。
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