烷基化哺乳动物DNA中磷酸三酯的碱性水解
The alkaline hydrolysis of phosphotriesters in alkylated mammalian DNA.
作者信息
Crathorn A R, Shooter K V
出版信息
Biochim Biophys Acta. 1982 May 31;697(2):259-61. doi: 10.1016/0167-4781(82)90086-0.
PMID:7104358
Abstract
Isolated DNA was alkylated with N-[14C]methyl-N-nitrosourea or N-[14C]ethyl-N-nitrosourea. Sedimentation analysis of the alkylated DNA before and after alkaline hydrolysis was used to determine the number of single-strand breaks introduced by hydrolysis of the triesters. Vacuum distillation from alkylated DNA solutions before and after alkaline hydrolysis was used to determine the numbers of triesters hydrolysing to the alcohol.
摘要
分离出的DNA用N-[¹⁴C]甲基-N-亚硝基脲或N-[¹⁴C]乙基-N-亚硝基脲进行烷基化处理。通过对碱性水解前后烷基化DNA的沉降分析来确定三酯水解引入的单链断裂数。通过对碱性水解前后烷基化DNA溶液的真空蒸馏来确定水解为醇的三酯数量。
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