Garrido-Pertierra A, Cooper R A
FEBS Lett. 1983 Oct 17;162(2):420-2. doi: 10.1016/0014-5793(83)80799-6.
A strain of Escherichia coli K-12 defective in pyruvate kinase F has been produced. The existence of this mutant, in conjunction with earlier results, strongly suggests that the two pyruvate kinases in this bacterium are distinct forms and not interconvertible. Either form of pyruvate kinase appeared to be equally effective in the glycolytic conversion of phosphoenolpyruvate to pyruvate. Genes specifying pyruvate kinase A and pyruvate kinase F were present on the small F-prime F506 and the locus for pyruvate kinase F was found to be at minute 36.5 on the E. coli genetic map.
已产生一株丙酮酸激酶F缺陷型的大肠杆菌K-12。该突变体的存在，结合早期的研究结果，有力地表明该细菌中的两种丙酮酸激酶是不同的形式，且不能相互转化。两种形式的丙酮酸激酶在磷酸烯醇丙酮酸糖酵解转化为丙酮酸的过程中似乎同样有效。编码丙酮酸激酶A和丙酮酸激酶F的基因存在于小F-prime F506上，并且发现丙酮酸激酶F的基因座在大肠杆菌遗传图谱的36.5分钟处。
