来自大肠杆菌的两种丙酮酸激酶同工酶结构基因的克隆：这些酶在丙酮酸生物合成中的相对作用。
Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.
作者信息
Ponce E, Flores N, Martinez A, Valle F, Bolívar F
机构信息
Departamento de Microbiología Molecular, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos.
出版信息
J Bacteriol. 1995 Oct;177(19):5719-22. doi: 10.1128/jb.177.19.5719-5722.1995.
Abstract
We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism. These genes were insertionally inactivated with antibiotic resistance markers and utilized to interrupt one or both pyk genes in the E. coli chromosome. With these constructions, we were able to study the role of these isoenzymes in pyruvate biosynthesis.
摘要
我们报道了从大肠杆菌中克隆出pykA和pykF基因，这两个基因编码该微生物中的两种丙酮酸激酶同工酶（ATP：丙酮酸2-O-磷酸转移酶；EC 2.7.1.40）。这些基因用抗生素抗性标记进行插入失活，并用于中断大肠杆菌染色体中的一个或两个pyk基因。利用这些构建体，我们能够研究这些同工酶在丙酮酸生物合成中的作用。
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