氰环素A对原核细胞和真核细胞核酸生物合成的抑制作用。

Inhibition of nucleic acid biosynthesis in procaryotic and eucaryotic cells by cyanocycline A.

作者信息

Hayashi T, Okutomi T, Suzuki S, Okazaki H

出版信息

J Antibiot (Tokyo). 1983 Sep;36(9):1228-35. doi: 10.7164/antibiotics.36.1228.

DOI:10.7164/antibiotics.36.1228

Abstract

Cyanocycline A was cytotoxic against Meth A cells in vitro, and also showed marked activity against the same cell line grown as an experimental ascites tumor. This antibiotic inhibited nucleic acid synthesis in Escherichia coli and Meth A cells. The antimicrobial activity of the antibiotic was reversed by addition of exogenous herring sperm DNA. Cytofluorometric analysis of cyanocycline A-treated Meth A cells showed an unusual pattern of the relative content of DNA and RNA per cell. These results suggested that cyanocycline A binds to DNA, and that it inhibits nucleic acid synthesis.

摘要

环青霉素A在体外对Meth A细胞具有细胞毒性，并且对作为实验性腹水瘤生长的同一细胞系也显示出显著活性。这种抗生素抑制大肠杆菌和Meth A细胞中的核酸合成。添加外源鲱鱼精DNA可逆转该抗生素的抗菌活性。对经环青霉素A处理的Meth A细胞进行细胞荧光分析，显示每个细胞的DNA和RNA相对含量呈现出异常模式。这些结果表明，环青霉素A与DNA结合，并抑制核酸合成。