Omura S, Otoguro K, Tanaka H
J Antibiot (Tokyo). 1983 Dec;36(12):1755-61. doi: 10.7164/antibiotics.36.1755.
The mode of action of virustomycin A, a novel 18-membered macrolide, on Trichomonas foetus was investigated. The antibiotic inhibited the biosynthesis of RNA, DNA and protein in the organism. The inhibition of RNA biosynthesis was the most severe. Virustomycin A repressed the incorporation of [3H]uridine into both acid-soluble and insoluble fractions, whereas actinomycin D inhibited the incorporation of [3H]uridine into acid-insoluble fraction alone. Furthermore, it was found that virustomycin A interfered with nucleotide formation from uridine and adenosine but not with their transport to the cells. On the other hand, the antibiotic did not inhibit the activities of uridine kinase and uracil phosphoribosyltransferase in a cell-free extract from the organism. These data suggest that the antibiotic interferes with the formation of phosphate donor(s) (possibly ATP-forming system) of the organism.
研究了新型18元大环内酯类抗生素维鲁斯霉素A对胎儿三毛滴虫的作用方式。该抗生素抑制了该生物体中RNA、DNA和蛋白质的生物合成。对RNA生物合成的抑制最为严重。维鲁斯霉素A抑制[3H]尿苷掺入酸溶性和不溶性部分，而放线菌素D仅抑制[3H]尿苷掺入酸不溶性部分。此外，发现维鲁斯霉素A干扰了尿苷和腺苷形成核苷酸的过程，但不影响它们向细胞的转运。另一方面，该抗生素在该生物体的无细胞提取物中不抑制尿苷激酶和尿嘧啶磷酸核糖转移酶的活性。这些数据表明，该抗生素干扰了该生物体中磷酸供体（可能是ATP形成系统）的形成。
