Staudenbauer W L
Eur J Biochem. 1976 Mar 1;62(3):491-7. doi: 10.1111/j.1432-1033.1976.tb10183.x.
Oxolinic acid, a quinolone antibacterial agent, inhibits reversibly the ATP-dependent replicative DNA synthesis in permeable cell systems as well as in cellophane disk lysates. It is about 10-fold more active than the structurally related nalidixic acid. Both drugs have no effect on the ATP-independent DNA repair, but interfere to some extent with RNA synthesis in permeable cells. They appear to interact with the same target since spontaneous nalidixic-acid-resistant mutants of nalA phenotype are also resistant to oxolinic acid. Full sensitivity to oxolinic acid can be conferred to lysates from resistant cells by addition of extracts from sensitive cells.
恶喹酸是一种喹诺酮类抗菌剂，在可渗透细胞系统以及玻璃纸圆盘裂解物中可可逆地抑制ATP依赖性复制性DNA合成。其活性比结构相关的萘啶酸高约10倍。两种药物对不依赖ATP的DNA修复均无作用，但在一定程度上会干扰可渗透细胞中的RNA合成。它们似乎与同一靶点相互作用，因为nalA表型的自发萘啶酸抗性突变体对恶喹酸也具有抗性。通过添加敏感细胞提取物，可使抗性细胞裂解物对恶喹酸完全敏感。
