Hochberg Z, Perlman R, Brandes J M, Benderli A
J Clin Endocrinol Metab. 1983 Dec;57(6):1311-3. doi: 10.1210/jcem-57-6-1311.
We have described a human term trophoblast cell culture system which synthesizes hormones de novo from their precursors. In the present report we utilized this monolayer system to show that insulin produced a dose-dependent stimulation of hPL secretion, with a significant effect at 10(-10) M insulin. At 10(-9) M concentration, insulin also inhibited estradiol secretion by the cultured trophoblast. We conclude that insulin regulates secretion of estradiol and placental lactogen by cultured human term trophoblast.
我们已经描述了一种人足月滋养层细胞培养系统，该系统能从其前体物质中重新合成激素。在本报告中，我们利用这个单层细胞系统来表明胰岛素对人胎盘催乳素（hPL）的分泌产生了剂量依赖性刺激，在胰岛素浓度为10^(-10) M时具有显著作用。在10^(-9) M浓度下，胰岛素还抑制了培养的滋养层细胞分泌雌二醇。我们得出结论，胰岛素可调节培养的人足月滋养层细胞分泌雌二醇和胎盘催乳素。
