Perlman R, Barzilai D, Bick T, Hochberg Z
Mol Cell Endocrinol. 1985 Nov;43(1):77-82. doi: 10.1016/0303-7207(85)90044-9.
The effect of insulin in physiological concentrations on hormone secretion by human term trophoblast cell culture was studied in relation to insulin control of glucose transport and utilization. Specific inhibitors to these 2 functions were added to the culture, either alone to test for a dose response or with insulin. 2-Deoxy-D-glucose, which inhibits glycolysis, did not change the pattern of response of estradiol and placental lactogen (hPL) secretion to insulin. Phloridzin, an inhibitor of glucose transport, interfered with the stimulatory effect of insulin on hPL secretion, but stimulated insulin inhibition of estradiol secretion when added by itself. The results indicate that hPL response to insulin is dependent on glucose transport, but is independent of glycolysis. Estradiol response is dependent on glucose transport, but is independent of glycolysis.
研究了生理浓度的胰岛素对人足月滋养层细胞培养物激素分泌的影响，并将其与胰岛素对葡萄糖转运和利用的控制联系起来。针对这两种功能的特异性抑制剂被添加到培养物中，单独添加以测试剂量反应，或与胰岛素一起添加。抑制糖酵解的2-脱氧-D-葡萄糖并未改变雌二醇和胎盘催乳素（hPL）分泌对胰岛素的反应模式。葡萄糖转运抑制剂根皮苷干扰了胰岛素对hPL分泌的刺激作用，但单独添加时会刺激胰岛素对雌二醇分泌的抑制作用。结果表明，hPL对胰岛素的反应依赖于葡萄糖转运，但与糖酵解无关。雌二醇的反应依赖于葡萄糖转运，但与糖酵解无关。
