Hochberg Z, Bick T, Perlman R, Lahav M, Barzilai D
Mol Cell Endocrinol. 1984 Oct;37(3):359-62. doi: 10.1016/0303-7207(84)90106-0.
Previous reports have documented the negative regulatory effect of calcium ion on the secretion of human placental lactogen by the placenta. Human term trophoblasts were dispersed from term placenta and maintained in monolayer cell culture. Incubation of the cultured trophoblast with EGTA produced a dose-dependent stimulation of placental lactogen secretion. The calcium ionophore A23187 inhibited hormone secretion. This inhibitory effect was eliminated by the addition of the calcium-calmodulin complex inhibitor trifluoperazine. It is concluded that calcium exerts a negative regulatory effect on the secretion of placental lactogen by a mechanism which requires the action of the calcium-calmodulin complex.
以往的报告已证明钙离子对胎盘分泌人胎盘催乳素具有负调节作用。足月人滋养层细胞从足月胎盘分离出来，并维持在单层细胞培养中。用乙二醇双四乙酸（EGTA）孵育培养的滋养层细胞会产生剂量依赖性的胎盘催乳素分泌刺激作用。钙离子载体A23187抑制激素分泌。添加钙调蛋白复合物抑制剂三氟拉嗪可消除这种抑制作用。得出的结论是，钙通过一种需要钙调蛋白复合物作用的机制对胎盘催乳素的分泌发挥负调节作用。
