Kelly M T, Buck G E, Fojtasek M F
J Clin Microbiol. 1983 Sep;18(3):554-7. doi: 10.1128/jcm.18.3.554-557.1983.
An in-use evaluation of a commercially available lysis-centrifugation blood culture system (Isolator; Du Pont Co., Wilmington, Del.) is presented. The Isolator was compared with biphasic bottles containing Trypticase soy broth and agar for the detection of organisms in 3,129 paired blood samples. Of 272 potential pathogens recovered, 78% were detected by the Isolator system, and 69% were detected by the biphasic bottle. A total of 31% of these organisms were detected only by the Isolator, and 22% were detected only by the biphasic bottle. The Isolator demonstrated enhanced detection of facultative gram-negative bacilli, anaerobic bacteria, and polymicrobial cultures. The biphasic bottle was more effective for the recovery of facultative gram-positive cocci, especially Streptococcus pneumoniae. The two systems were equally effective for the recovery of yeasts. Contamination rates were 3% for the Isolator and 3.2% for the biphasic bottle. The results indicate that the Isolator system performs well in routine clinical use, but it should be complemented by another method to obtain optimal detection of bacteremia. The biphasic bottle provides an acceptable complementary system both in terms of utility and performance.
本文介绍了一种市售的裂解离心血液培养系统(Isolator;杜邦公司,特拉华州威尔明顿)的使用评估。将Isolator与含有胰蛋白酶大豆肉汤和琼脂的双相瓶进行比较,以检测3129对配对血液样本中的微生物。在回收的272种潜在病原体中,Isolator系统检测到78%,双相瓶检测到69%。这些微生物中,共有31%仅由Isolator检测到,22%仅由双相瓶检测到。Isolator在兼性革兰氏阴性杆菌、厌氧菌和多种微生物培养物的检测方面表现出增强的效果。双相瓶在兼性革兰氏阳性球菌,尤其是肺炎链球菌的回收方面更有效。两种系统在酵母回收方面同样有效。Isolator的污染率为3%,双相瓶的污染率为3.2%。结果表明,Isolator系统在常规临床使用中表现良好,但应辅以另一种方法以获得最佳的菌血症检测效果。双相瓶在实用性和性能方面都提供了一个可接受的补充系统。