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快速识别携带大量B族链球菌的孕妇。

Rapid identification of pregnant women heavily colonized with group B streptococci.

作者信息

Jones D E, Friedl E M, Kanarek K S, Williams J K, Lim D V

出版信息

J Clin Microbiol. 1983 Sep;18(3):558-60. doi: 10.1128/jcm.18.3.558-560.1983.

Abstract

Pregnant women admitted to Tampa General Hospital, Tampa, Fla., were cultured for group B streptococci (GBS). Culture swabs were placed into enriched, selective Todd-Hewitt medium and were quantitated for GBS. The broth cultures were tested by slide coagglutination before incubation and after 5 and 20 h of incubation. Fifty-four (27%) of the 201 maternity patients cultured were positive for GBS and were identified as such by slide coagglutination. A strong correlation was found between the magnitudes of colonization and the times required to identify the broth cultures as GBS positive. Cultures from mothers heavily colonized (mean concentrations of 3 X 10(4) GBS per culture swab or greater) were identified after 5 h or less of incubation. Mothers lightly colonized with GBS (mean concentrations of 2 X 10(2) GBS per culture swab) were identified only after their broth cultures had been incubated for 20 h.

摘要

对入住佛罗里达州坦帕市坦帕综合医院的孕妇进行了B族链球菌(GBS)培养。将培养拭子放入富集的选择性托德-休伊特培养基中,并对GBS进行定量。肉汤培养物在培养前以及培养5小时和20小时后通过玻片凝集试验进行检测。在培养的201名产妇中,有54名(27%)GBS检测呈阳性,并通过玻片凝集试验得以确认。发现定植程度与将肉汤培养物鉴定为GBS阳性所需时间之间存在很强的相关性。来自重度定植母亲(每个培养拭子GBS平均浓度为3×10⁴或更高)的培养物在培养5小时或更短时间后即可鉴定出来。GBS轻度定植的母亲(每个培养拭子GBS平均浓度为2×10²)只有在其肉汤培养物培养20小时后才能鉴定出来。

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