来自鼠类劳氏肉瘤病毒的一株分离株在灵长类动物中的嗜异性特性

Xenotropic properties of an isolate from murine Rauscher leukemia virus in primates.

作者信息

Ablashi D V, Oie H K, Armstrong G R, Didier-Fichet M L, Tronick S R, Heine U, Dalton A J

出版信息

J Med Primatol. 1976;5(4):223-36. doi: 10.1159/000459953.

Abstract

Murine Rauscher leukemia virus (MuRLV) from BALB/c plasma consisting of a mixture of an ecotropic and a xenotropic virus could be separated out by a selection process when propagated in human and simian cell cultures. This hypothesis is supported by obtaining consistently lower infectivity titers of human cell propagated RLV in human and simian cells as compared to MuRLV propagated in mouse cell cultures. Furthermore, RLV passaged in a simian cell culture failed to replicate in mouse cells, had a wide host range, was able to rescue Moloney sarcoma genome, possessed murine type C group-specific antigen, and was neutralized by anti-HRLV. Its reverse transcriptase was strongly inhibited by antiserum to MuRLV enzyme; however, antiserum to woolly monkey enzyme also inhibited (30%) its reverse transcriptase, suggesting some difference in antigenic properties. Inoculation of this virus in rhesus monkeys was inconclusive.

摘要

来自BALB/c血浆的鼠类劳氏肉瘤病毒（MuRLV）由嗜亲性病毒和异嗜性病毒的混合物组成，当在人及猴细胞培养物中传代时，可通过选择过程将其分离出来。这一假设得到了以下证据的支持：与在小鼠细胞培养物中传代的MuRLV相比，在人细胞中传代的RLV在人和猴细胞中的感染性滴度始终较低。此外，在猴细胞培养物中传代的RLV在小鼠细胞中无法复制，具有广泛的宿主范围，能够拯救莫洛尼肉瘤基因组，拥有鼠类C型群特异性抗原，并能被抗-HRLV中和。其逆转录酶受到抗MuRLV酶血清的强烈抑制；然而，抗绒毛猴酶血清也能抑制（30%）其逆转录酶，这表明其抗原特性存在一些差异。将这种病毒接种到恒河猴中，结果尚无定论。