一种在复制和肉瘤病毒辅助功能方面对温度敏感的哺乳动物白血病病毒突变体的热不稳定逆转录酶。
Thermolabile reverse transcriptase of a mammalian leukemia virus mutant temperature sensitive in its replication and sarcoma virus helper functions.
作者信息
Tronick S R, Stephenson J R, Verma I M, Aaronson S A
出版信息
J Virol. 1975 Dec;16(6):1476-82. doi: 10.1128/JVI.16.6.1476-1482.1975.
Abstract
Three temperature-sensitive mutants of the Rauscher strain of murine leukemia virus are defective in early post-penetration functions required both for leukemia virus infection and for initiation of transformation of cells by their pseudotypes of murine sarcoma virus. In the present study, the reverse transcriptase of one of these mutants (ts 29) is shown to be thermolabile compared with the enzymes of the wild-type virus and several other temperature-sensitive mutants. These findings provide evidence that the reverse transcriptase is required both for leukemia virus infection and for initition of transformation by the replication-defective murine sarcoma virus genome.
摘要
劳氏鼠白血病病毒的三个温度敏感突变体在穿透后早期功能上存在缺陷，这些功能对于白血病病毒感染以及通过其鼠肉瘤病毒假型引发细胞转化都是必需的。在本研究中，与野生型病毒和其他几个温度敏感突变体的酶相比，其中一个突变体（ts 29）的逆转录酶表现出热不稳定。这些发现提供了证据，表明逆转录酶对于白血病病毒感染以及复制缺陷型鼠肉瘤病毒基因组引发的转化都是必需的。
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本文引用的文献
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Separation of murine cellular and murine leukaemia virus DNA polymerases.小鼠细胞和小鼠白血病病毒DNA聚合酶的分离
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